New Mexico Molecular Libraries Screening Center

The New Mexico Molecular Libraries Screening Center is part of the Molecular Libraries Initiative within the overall NIH Roadmap.

Project Overview

PI: SKLAR, LARRY A. (lsklar at salud.unm.edu)
Grant Number: 1U54MH074425-01PI
Project Title: New Mexico Molecular Libraries Screening Center
Abstract:
We have developed innovative flow cytometric tools for discovery research that enable homogeneous analysis of ligand binding and protein-protein interaction, HT sample handling, high content analysis, and real-time measurements of cell response. more...

New Mexico MLSC Inventory/Capabilities

Space

The NM MLSC occupies and has available the following contiguous space on the second floor of UNM Cancer Research Facility:

• 2 x 650 sq ft laboratories (CRF219 and 221) for sample preparation and physical screening
• 200 sq ft of laboratory space for assay development and implementation (CRF 229)

Equipment

1. Assay Development / Adaptation (AD/A):

• 2 custom designed HyperCyt® high-throughput flow cytometry instrument clusters for sample delivery from microplate to flow cytometer with custom software to control operational and analytical functions
• 4 BD Biosciences FACScan Flow Cytometers dedicated to assay development

2. High Throughput Screening / Implementation (HTS/I):

• 3 HyperCyt® HT flow cytometry instrument clusters for sample delivery from microplate to flow cytometer with custom software to control operational and analytical functions
• 1 Accuri® C6 benchtop flow cytometer; 2 laser / 4 fluorescence parameters / 2 light scatter detectors

Center Expertise

Cellular Assays:

G protein-coupled receptors; Integrins and adhesion molecules; Proteases/proteasome; Cell cycle; Apoptosis

Bead-based assays:

Protein-protein interactions; Protein-nucleic acid interactions; Kinase assays

Informatics:

State-of-the-art, innovative tools for ligand-based and structure-based virtual screening; Unique tools for post-HTS analyses, clustering and in silico profiling; High performance tools for bioinformatics analyses; Unique data archiving and data mining capabilities; Tools to evaluate post-HTS analyses

Capacity

Projected: 5,000-10,000 wells per day per detector

MOLECULAR LIBRARIES INITIATIVE - SCREENING CENTERS

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